The genetic structure of F 2 sporophytic populations after F 1 sunflower pollen storage at low temperature has been studied. Freshly collected pollen was stored at the temperature of 3±1°C for a period of 7'8 days and used to self-pollinate the emasculated F 1 plants. F 2 seeds were germinated at 5±1°C and then the percentage of seed germination was counted. Germinated and not germinated seeds were separately planted in the phytotron at an optimum temperature. Segregation ratios in F 2 populations for marker traits were analyzed at the stage of the second pair of true leaves. Pollen treatment compared with the control (fresh pollen) significantly changed in F 2 populations monogenic ratios for some marker traits. In some cross combinations increase in the cold tolerance of F 2 populations was found. Obtained results show that pollen storage at low temperature selectively influences the male gametophytes of F 1 hybrids that change the genetic structure of F 2 populations.