The objective of the study was to provide new genetic variability in important agronomic traits that can be exploited for improvement of sunflower production. Seeds of eight sunflower inbred lines from gene collection of Institute of Field and Vegetables Novi Sad were irradiated with gamma rays (γ) and fast neutrons (Nf) and treated in ethyle-methane-sulphonate (EMS) solution. The manifestation of mutations was mostly expressed in M 2 and M 3 generation. Seven mutants were developed; one early flowering two short stature and one high two with higher oil content and one branching. The stable progenies were evaluated in micro-plot tests in M 6 generation for seed yield and other traits in comparison with respective original line. Further studies should be focused on testing new mutant lines in hybrid combinations as well as the determination of inheritance of mutant traits.