The sunflower is grown widely for the purpose of edible oil. The oil is most desirable for its oil content and quality because it contains a number of polyunsaturated fatty acids (oleic acid and linoleic acid) as well as saturated fatty acids (palmitic acid and stearic acid). Oils with more unsaturated fatty acids are preferred as they lower the cholesterol content in a human body. High oleic sunflower oil has a higher oxidative stability than most other cooking oils and they are most effective in preventing cardio vascular diseases. Hence an experiment was conducted with a major objective to evaluate diverse germplasm lines for high oil content and high oleic acid and to identify molecular markers for high oleic acid. The genotypes included cms -lines R-lines germplasm lines and inbreds. Initially the selected genotypes were screened with SSR markers. Among them a few primers showed differentiating bands between low and high oleic contents. To confirm the primer the initial study was carried out with the released hybrids. Simultaneously fatty acid profiling was also done for four fatty acids using gas chromatography-mass spectroscopy. Oil content was also estimated in these genotypes by NMR spectrometer. The range for oil content was from 28% to 44% whereas for oleic acid it was from 23.63% to 90.58%. Later the primer was used for further screening of all the genotypes and the parental lines were selected for the heterosis breeding programme.