Interspecific hybridization was carried out between cultivated sunflower Helianthus annuus L. and accessions from perennial wild species of genus Helianthus with the aim to develop hybrid plants with enriched heritability and higher resistance to abiotic and biotic factors. In order to overcome the difficulties in the process of crossing, the embryo rescue method was applied. Two CMS lines developed at Dobrudzha Agricultural Institute – General Toshevo (DAI) were included as mother components in hybridization. Accessions from five perennial wild species of genus Helianthus with different ploidy levels were used as male components; these wild species are being maintained in the wild sunflower collection of DAI. Ten crosses were made. Thirty-eight embryos were obtained, which were cultivated on nutrition medium. Six F1 interspecific hybrids were produced, differing by their habit type. All obtained interspecific hybrids were sterile but were able to plant propagate vegetatively.