As many as 251 insect and acarid species are known to attack sunflower crop worldwide. Recently, a stem borer has emerged as a new and regular pest of sunflower in Marathwada region (M.S.) India. It is a coleopterous grub Nupserha sp. near vexator (Pascoe), which belongs to the family Cerambyci dae: Lamiinae. Hence, the screening of sunflower germplasm lines was con ducted during last five years to identify resistance sources against stem borer for further exploitation in breeding programs. A set of 100 germplasm lines was annually received from All-India Coordinated Research Project on Sunflower during 2002-2006 to evaluate sources of resistance for major sunflower pests. The screening work was carried out at Oilseeds Research Station, Latur, M.S., India, which was identified as a hotspot for stem borer in sunflower. Reactions to stem borer received from the germplasm lines were categorized for ease in evaluation of resistance potential of each test line. Stem borer inci dence varied from free to >80% in the germplasm under investigation during last five years indicating the presence of adequate variability in the material for their response to stem borer infestation.