The target region amplification polymorphism (TRAP) marker technique was employed to expand the published sunflower simple sequence repeat (SSR) linkage map constructed from a recombinant inbred population derived from the cross of RHA 280 × RHA 801. A previous report described the mapping of 183 TRAP markers generated by using fixed primers designed against the conserved Arabidopsis-type telomere sequence repeat into the above mentioned map of 577 SSR markers. Thirty-two markers were mapped to the outermost positions of the linkage groups, defining 21 of the 34 linkage group ends. This paper reports the integration of an additional 220 TRAP markers onto the same map. These newly added TRAP markers were generated by 23 combinations with fixed primers designed against selected sunflower ESTs showing homology with components of plant disease resistance genes and homeobox genes. The resulting map consists of 980 markers in 17 linkage groups (LG) and the total length is 1920 centiMorgan (cM). Although the aver age distance between two markers is less than two cM, there are four gaps larger than 20 cM (one in LG2, one in LG4, and two in LG6). The gaps could be due to the lack of polymorphism between the two parental lines in these chromosome regions with respect to the type of markers used in this study.