The experiment was conducted in order to study the regeneration potential of cotyledon explants from some Iranian inbred lines of sunflower (Helian thus annuus L.) and their F1 hybrids. Cotyledons of 2-day-old seedlings of three cytoplasmic male sterile inbred lines used as female (CMS 24, CMS 31 and CMS 60/52) and five inbred lines used as male (R-81.1, R-92, R-97, R-201 and R-232) and finally their 15 F1 hybrids were plated on shoot induction medium, Murashige and Skoog (MS) containing 3% sucrose, 0.5% agar-agar and different concentrations of BAP and NAA. Analysis of variance showed significant differences for all organogenesis traits studied among parental geno types and F1 hybrids, hormone combination treatments and genotype × hormone combination treatment interaction. Shoot organogenesis was optimized on MS medium with 1 mg l-1 BAP and 1 mg l-1 NAA. Interaction “(CMS 24 × R-201) × T2” showed the highest values for number of explants shooting per 100 explants plated (55%). Interaction “R-92× T2” showed the highest value for average number of shoots per explants plated (8.65). Interaction “R-81.1 × T2” showed the highest value for average number of shoots per explants shooting (23). Interaction “CMS 24 × T2” showed the highest value for number of explants rooting per 100 explants plated (35%).