Morphological variability between accessions of H. tuberosus collected in Montenegro and the USA was investigated as well as possible effect of long term cultivation or its absence and growing in different environmental conditions on morphological traits of this species. Results obtained showed that there is a large similarity between some populations collected in Montenegro and those collected in the USA. This, along with the presence of a great cluster formed exclusively from Montenegrin populations, supports the hypothesis that there were two ways of introduction of H. tuberosus to Montenegro. In a group of populations, the changes in morphology were found, probably due to absence of cultivation.