The genetics of anodal esterase (Est), cathodal esterase (cEst), cathodal acid phosphatase (cAcp) and malate dehydrogenase (Mdh) has been studied in mature seeds and leaves (genetics of cAcp and Mdh has not been studied in leaves) of sunflower (Helianthus annuus L.). A total of ten loci (four loci of anodal esterase, two loci of cathodal esterase, three loci of malate dehydrogenase and one locus of cathodal acid phosphatase) have been identified and described. Five esterase loci (Est1, Est2, Est3, Est4, cEst5), three malate dehydrogenase loci and one locus of cathodal acid phosphatase are expressed in seeds. Three esterase loci (Est2, cEst5 and cEst6) are expressed in leaves. The analysis of linkage between these loci has been made. Two linkage groups have been found. The sequence of the loci in the first linkage group was Mdh2-Est1-Est2-Est3-cEst5. In the second linkage group it was Est4-cAcp1. Linkages have been analyzed between three isoenzymatic loci expressed in leaves and between two loci controlling morphological traits (branched stem and male fertility restoration). The linkage between morphological traits and isoenzymatic loci has not been revealed. It has been revealed in Br-Rf pair.