Morphological variability of two Heltanthus species, H.giganteus and H.maximillani (section Divaricatt), was assessed at the Institute for Field and Vegetable Crops, Novi Sad. Fifteen populations of each species, with five plants per population, and thirty characters for each plant were analyzed. Three H.giganteus populations were found to differ morphologically in relation to the typical populations. Population 78 differed in the number of leaves per stem, length of involucral bracts and the length of ray flowers: population 2014 differed in leaf color and width and the angle between lateral veins and the midrib: population 2018 differed in stem color and the dentation of leaf margin. These populations originated from the western boundary of the distributional range of H.giganteus where species variability is high, representing specific adaptations that play an important role in the evolution of the species.