Twenty perennial and annual wild species from Hellanthus genus were Included in the research. Thirteen of them were diploid, three were tetraplold and four were hexaploid. Among the diploid species, four were annuals and

Four types of nutrient media with different NAA and BAP concentrations were used for callus Induction. In the diploid species, callus formation varied from 0.6 to 48.9%, in the tetraploid from 16.7 to 38.3%, and in the hexaploid nine were perennials. from 0.1 to 33.3%.

The variation in the perennial diploid species was from 0.6 to 48.9%, and in the annual from 17.2 to 26.7%.

Two variants of nutrient medla were used for regeneration from the obtained embriogenic calli - a medium with growth regulators and a medium without hormones. For primary regeneration. the medium with growth regulators gave a better response, while for the secondary regeneration reaction was positive on the hormone-free medium.

Regeneration was obtained in four of the studied species - H.mollts (M-020), H.mollis (M-034), H.salicifoltus (M-045) and H.smithit (M-008). The initial reaction in the species M-020 has begun from 3 embryogenic calli, In M-034 from 2 calli, in M-045 from 3 calli, and in M-008 from 5 calli, the numbers of obtained plants being 28. 38, 49 and 51, respectively.

The ploidy level of the obtained regenerants was determined floweytometrically.