Plant regeneration potential of two F1 sunflower interspecific hybrids was evaluated. Immature embryos (8-10 days old) were harvested. Basal MS medium supplemented with 2 mg/ml zeatin proved to be better than other variants tested for callus initiation and regeneration. Regenerable calli were obtained from both hybrids. Complete plantlets were formed within 20-30 days. Part of the regenerated plants showed no major alterations in morphology as compared with the original ones.