Sunflower plants (Helianthus annuus L. cv. NK-265) were grown hydroponically in half strength Hoagland nutrient solution (pH 5.7) to study the response of plant parts to graded levels of S (0, 10, 20. 30. 40 and 50 ug ml-1) in triplicate. Twelve-day old seedlings were transplanted. The harvests took place on the 29th and 36th day after transplanting. After harvesting, plant parts were separated into leaf, stem and root. Plant parts were dried in an oven at 70+2℃ after recording fresh weight. The samples were digested in HNO3 and HCIO4 and S was estimated. The application of 20 ug S ml^-1 significantly increased the root and shoot length. The relative growth rate (RGR) of plants grown in 20 ug S ml^-1 was 0.26 mg mg-^1 day^-1 as compared with 0. 188 mg mg^-1 day^-1 in case of the control. Similarly sulphur was much more efficiently used by plants grown in 20 ug S ml" than in the other treatments. Sulphur uptake in developing leaves (DL), expanded leaves (EL) and old leaves (OL) was curvilinearly correlated with the S applied. Sulphur uptake was increased by 40 and 480%, in DL and EL, respectively, when plants were grown in 20 ug S ml^-1. As the S application increased beyond 20 ug ml^-1, the uptake declined. Developing. older and expanded leaves responded clearly to the applied S. Roots acted as a supplier from the source to sink, stem responded lesser than leaves. As the metabolic activities are higher in expanded leaves, they showed a clear response.