Callus formation could be induced from segments of different organs prepared from mature seeds or germinated seedlings of sunflower. In the latter case, callus formation was pronounced from hypocotyl and cotyledon segments, howwer, no plants could be regenerated from such calluses. on the contrary, multiple shoot formation was achieved froir mature embryo segments and callus induced on these fragments. Genotypic differences were obvious for all types of response. Shoots could be rooted and induced to dwelop complete plants which could be grown to maturity. As a major obstacle, excessive "prematuie flo*'eringn must be avoided in order to improve the efficiency of vegetative propagation in vitro.