Prior to a treatment with GAs, biochemical characteristics of seed were analyzed in the sterile plants which were subsequently pollinated with pollen of the same line.It was found that the 1000-seed weight was reduced in the treated plants, while the kernel quantity remained unchanged. The kernel oil content and the protein quantity changed in dependence of genotype and vegetation conditions. The fatty acid composition of oil was also changed.It was concluded that the biochemical characteristics of F1 hybrid seeds produced after the sterilization of female plants with GA3 are not satisfactory. Similar investigations will be conducted on the later generations.