Botrytis stalk and head rot was observed on three sunflower inbred lines under greenhouse and field conditions at NARC, Islamabad. The disease was characterized by rotting of the stalk and head with profuse production of conidiophores and conidia on grey to dark brown lesions. Based on the morphological and cultural characteristics, the causal organism was identified as Botrytis cinerea Pers. ex. Fr., which appears to be a new record on sunflower from Pakistan. The incidence of stalk rot was 25 and 30 percent in inbred lines HA-89 and RHA-296, respectively and 30 percent in CMS HA-89 line for head rot under greenhouse while in traces under field conditions,