Cold pretreated anthers with uninucleate microspores of KBSH_1, "BSH-l", and "Modern" genotypes of sunflower, under dark incubation. resulted in maximum callus induction (>85%). The callus frequency from anthers of "KBSH- l " (92.8Oo/o) and "BSH- I " (92.7jo/o) was almost similar in MS medium with 2 mg/ I 2, 4-D and 1 mg/l BA, while in "Modern, it was the highest on medium with I mg/l each of NAA and BA. Uninucleate microspores of anthers are divided symmetrically to give rise to multicellular proembryo-like bodies. Occasional shoot bud formation from cultured anthers of "BSH-1" and "KBSH-I" was observed on medium with 0.5 or 1.0 mg/l NAA and 1.0 mg/l BA. Calli of "BSH-I" subcultured on plain MS medium resulted in the formation of meristematic centres.