Lysine content in sunflower seed storage protein is not sufficient to meet the requirements of FAO. As far as the storage protein amino acid composition of Helianthus annuus is concerned, the lysine content of the species from the IWS "Dobroudja" collection investigated in our laboratory is not substantially different from that of cultivated Helianthus annuus varieties and hybrids. In a previous study, we have reported data about Bidens tripartita's high lysine content in the storage protein of seeds (3.4%for Hetianthtts o.nrtuu.s and 5.8 g/lbr Brdens tripartita). In 1994, we collected 14 new Bidens tripartita accessions from different parts of Bulgaria. A biochemical characterization of these genotypes, including fatty acid composition, storage protein electrophoretic subunits, and amino acid composition, is presented in this study. It was established that linoleic acid varied from 64.0 % to 73.8% and oleic acid from 18.3% to 23.4%. Seven different storage protein electrophoretic patterns were found in the 14 investigated genotypes. Lysine content varied from 4.83% to 5.45%.