Protein content in seed and amino acid composition of proteins were studied in 30 genotypes of 16 Helianthus species. The results indicate that some entries of wild Helianthus can be used as a source of genes for improving the protein nutrient value in modern cultivars and hybrids.The entriesl H. hirsutus- M007, H.rigidus-M002 and M 028, H.mollis-M033,and H. eggerttii-M 001 were distinguished for a much higher protein content as compared with cv. Peredovik. Compared with the standard the entries H. annuus-E 004, H.petiolaris-E 021 and E 037, H. praecox-E 027, H. laevigatus-M 016, H. egertti- M 001, and H. rigidus-M 002 had increased contents of some amino acids. These entries are maintained at the Dobroudia IWS and can be used for wide hybridization of sunflower.