Research article | Open Access
Helia 1995, Vol. 18(22) 45-50
pp. 45 - 50
Publish Date: June 06, 1995 | Single/Total View: 0/0 | Single/Total Download: 0/0
Abstract
Attempts were made in sunflowers to develop plants from immature embryos instead of planting seed. Immature embryos of 12 days after flowering were dissected from seed- grown plants (SGP), and 1st cycle of embryo-raised plants (ERP) was obtained. When these plants were in the flowering stage, 12-day-old embryos were dissected, and 2nd cycle of ERP was raised. This procedure was followed for 5 cycles. Through this procedure, within 316 days, 5 cycles of ERP were completed. No variation was observed in plants of all 5 cycles of ERP except for male sterility. This methodology could be useful in the rapid development of inbred lines, fast conversion of inbred to cytoplasmic male sterile, or for any backcrossing programme as compared with the seed-to-seed cycle. Such rapid cycling of successive generations within a short period helps to achieve homozygosity early, which would accelerate the breeding cycle of crop plants.
Keywords: Sunflower, immature embryo, seed-grown plant (SGP), embryo-raised plant (ERP), cytoplasmic male sterility (CMS)
APA 7th edition
Jambhulkar, S.J. (1995). RAPD CYCLING THROUGH IMMATURE EMBRYO CULTURE IN SUNFLOWER (Helianthus annuus L.). Helia, 18(22), 45-50.
Harvard
Jambhulkar, S. (1995). RAPD CYCLING THROUGH IMMATURE EMBRYO CULTURE IN SUNFLOWER (Helianthus annuus L.). Helia, 18(22), pp. 45-50.
Chicago 16th edition
Jambhulkar, Sanjay J. (1995). "RAPD CYCLING THROUGH IMMATURE EMBRYO CULTURE IN SUNFLOWER (Helianthus annuus L.)". Helia 18 (22):45-50.