The research evaluates four methods of pollination: 1) bees, 2) open pollination, 3) hand polination using mixted pollen,4) self-pollination. The test included 11 populations of wild H. annuus and six populations of H. petiolais ssp. petiolaris. The method of controlled pollination with bees produced significantly higher numbers of achenes than the other three method in all but two. H. annuus populations. With H.petiolaris, the controlled bee pollination produced a lower numbers of achenes than the open pollination. After 50 days in cage, the bee swarms stopped multiplying, i.e., the queens stopped ovipositing and the workers threw eggs out of the cells. After the end of pollination, the swarms were fewer in number than at the beginning of pollination.